杜林方教授博导
职务:
浙江大学生物化学技术研究所所长
中国植物生理学会光合、固氮与代谢专委会副主任委员
国际光合作用研究会（ISPP）会员
浙江省生物化学与分子生物学学会副理事长
国家级重点学科植物学学科（植物生理方向）带头人
浙江省学术与技术带头人
任《Acta Biochem Biophys Sin》、《Prog Biochem Biophys》、《应用与环境生物学报》等刊物编委。
电话:028-85415008
Email:[email protected]
简历:
在浙江大学生物系生物化学专业获学士学位(1985年)和理学硕士学位（1988年）。
2001年浙江大学获理学博士学位。
1988年7月浙江大学生物系助教、讲师；1993年5月副教授；1995年11月至今教授
2003-2004美国特拉华大学、波士顿大学访问学者。
研究方向:
光合蛋白结构与功能、生物信息学、肿瘤抑制蛋白的结构与功能
研究内容:
植物光合作用光系统II结构与功能研究；蛋白质磷酸化与去磷酸化对其结构与功能调节机理研究；肿瘤抑制蛋白的结构与功能研究；蛋白酶的结构及催化反应机理研究；植物重要蛋白代谢调控的分子机理研究；蛋白质结构预测及分子设计；蛋白酶抑制剂的结构及其应用
从事科研项目:
国家重点基础研究发展计划（973计划）：光合作用分子机理及其在农业生产中应用的基础研究（2009CB118500）子课题：叶绿体蛋白的功能研究，2009-2013
国家自然科学基金项目“蛋白质乙酰化对光系统II结构与功能调节的分子机理”, 31170223, 2012-2015
国家“十一五”科技支撑计划重点项目“科研用生化试剂核心单元物质及共性关键技术的研制与开发”, 2006BAF07B01, 2007-2010
国家教育部博士点基金“菠菜叶绿体psbC基因原核表达、色素组装与功能的实现”, 2008-2010
国家自然科学基金项目“菠菜光系统II psbC基因的在酵母中异源表达、体外色素组装与功能的实现”, 30870181, 2009-2011
国家自然科学基金项目“光对植物光系统II反应中心D1蛋白编码基因表达的调控机理”, 2003-2005
国家自然科学基金项目“蛋白质磷酸化对植物光系统II反应中心功能的影响”, 2000-2002
发表SCI论文:
1.LyuLei, Whitcomb Elizabeth A., Jiang Shuhong, Chang Min-Lee, Gu Yumei, Duncan Melinda K., Cvekl Ales, Wang Wei-Lin, Limi Saima, Reneker Lixing W., Shang,Du Linfang*,*.Unfolded-protein response-associated stabilization of p27 (Cdkn1b) interferes with lens fiber cell denucleation leading to cataract. FASEB J. 2016, 30:1087-1095.(SCI, IF=5. 22)
2.ZhuGuo-fei, Wang Yu, Xi Lei, Liu Jin, Wang Hao,Du Lin-fang* Spectroscopy and molecular docking studies on the binding of propyl gallate to human serum albumin., Journal of Luminescence, 2015, 159: 188–196.(SCI, IF=2. 69)
3. Han Yong-Guang, Tan Qiu-Ju, Liu Ke, Liu Yang,Du Lin-Fang* Mercuric chloride damages the thylakoid membrane structure of chloroplasts and alters the profiles of SOD, CAT and EST isozymes inChlamydomonas reinhardtii. Fresenius Environmental Bulletin, 2015, 24: 676-682.(SCI, IF=0. 37)
4.Wang Jing-Zhang, Xi Lei, Lin Tao, Wang Yu, Zhu Guo-Fei,Du Lin-Fang* The structural and functional role of the three tryptophan residues in Pin1.. Journal of Photochemistry and Photobiology B: Biology 2015, 146: 58–67.(SCI, IF=3. 03)
5. Xiao QJ, Li ZG, Yang J, He Q, Xi L,Du LF* Heat-induced unfolding of apo-CP43 studied by fluorescence spectroscopy and CD spectroscopy.. Photosynth Res. 2015,126: 427-435.(SCI, IF=4. 12)
6.Wang Y, Zhao M, Zhang Q, Zhu GF, Li FF,Du LF* Genomic distribution and possible functional roles of putative G-quadruplex motifs in two subspecies ofOryza sativa. Comput Biol Chem.2015,56:122-130.(SCI, IF=1. 01)
7. Qie Beibei, Lyu Zhou, Lyu Lei, Liu Jun, Gao Xuejie, Liu Yanyan, Wei Duan, Zhang Nianhui,Du Linfang*, Liu Ke*. Sch9 regulates intracellular protein ubiquitination by controlling stress responses. Redox Biol. 2015, 5:290-300. (SCI, IF=6. 24)
8. Zhu Guo-Fei, Ren Si-Yan, Xi Lei, Du Lin-Fang*, Zhu Xiao-Feng*, Temperature induced structural transitions from native to unfolded aggregated states of tobacco etch virus protease. J Mol Struct 2015, 1082: 80–90. (SCI, IF=1. 78)
9. Yao Jie, Wu Jibing, Yang Xue, Yang Jiaxi, Zhang Yuxing, andDu Linfang*, Oleuropein Induced Apoptosis in HeLa Cells via a Mitochondrial Apoptotic Cascade Associated With Activation of the c-Jun NH2-Terminal Kinase. J Pharmacol Sci 2014, 125: 300–311. (SCI, IF=2. 36)
10. Wang Yu, Xi Lei, Yao Jie, Yang Jiao,Du Lin-Fang*.Role of pH in structural changes for Pin1 protein: an insight from molecular dynamics study.J Mol Model, 2014, 20: 2376 (SCI, IF=1. 79)
11. Liu Ji, Xie Si-Si, Luo Yue, Zhu Guo-Fei,Du Lin-Fang*.Soluble expression of Spinach psbC gene in Escherichia coli and in vitro reconstitution of CP43 coupled with chlorophyll a only. Plant Physiol Biochem. 2014, 79:19-24. (SCI, IF=2. 10)
12. Han Yongguang, Luo Yue, Qin Shirong, Xi Lei, Wan Bo,Du Linfang*, Induction of systemic resistance against tobacco mosaic virus by Ningnanmycin in tobacco. Pesticide Biochemistry and Physiology. 2014, 111:14-18. (SCI, IF=2. 01)
13. Zhu Guo-fei, Wang Yu, Liu Jin, Wang Hao, Xi Lei,Du Lin-fang* Interaction Between Ginkgolic Acid and Human Serum Albumin by Spectroscopy and Molecular Modeling Methods.. J Solution Chem, 2014, 43:1232-1249 (SCI, IF=1. 17)
14. Wang Jing-Zhang, Liu Ji, Lin Tao, Han Yong-Guang, Luo Yue, Xi Lei,Du Lin-Fang*. Aluminum (III) interferes with the structure and the activity of the peptidyl-prolyl cis-trans isomerase (Pin1): A new mechanism contributing to the pathogenesis of Alzheimer's disease and cancers? Journal of Inorganic Biochemistry, 2013, 126: 111-117 (SCI, IF=3. 27)
15. Yang Xue, Yao Jie, Luo Yue, Han Yong-guang, Wang Zuo-bai, andDu Linfang*, P38 MAP kinase mediates apoptosis after genipin treatment in non–small-cell lung cancer H1299 cells via a mitochondrial apoptotic cascade. J Pharmacol Sci. 2013, 121: 272-281(SCI, IF=2. 11)
16. Wang Yu, Zhu Guo-Fei, Ren Si-Yan, Han Yong-Guang, Luo Yue,Du Lin-Fang*. Insight into the structural stability of wild type and mutants of the tobacco etch virus protease with molecular dynamics simulations. J Mol Model, 2013, 19:4865-4875 (SCI, IF=2. 10)
17. Wang Jing-Zhang, Ren Si-Yan, Zhu Guo-Fei, Xi Lei, Han Yong-Guang, Luo Yue,Du Lin-Fang*, Hg2+interference with the structure of tobacco etch virus protease (TEVp) and its implications for biological engineering. J Mol Struct 2013, 1052: 85-92. (SCI, IF=1. 60)
18. Wang Jing-Zhang, Li Shu-Rui, Li Ya-Li, Zhang Yong-Ze, Zhang Ting, Zhao Chao-Xian, Yao Chun-Xia,Du Lin-Fang*, Could Pin1 help us conquer essential hypertension at an earlier stage? A promising early-diagnostic biomarker and its therapeutic implications for the disease.
Medical Hypotheses, 2013, 81: 931-935. (SCI, IF=1. 15)
19. Zhang Nian-Hui, Zhao Yun, Liang Hou-Guo, Sun Jie-Yin,Du Lin-Fang*. A new gun mutant of oilseed rape with a reduced porphyrin flux through Mg-chelatase.Acta Physiol Plant, 2013, 35: 75-84. (SCI, IF=1. 52)
20. Zhao Yun,Du Linfang*, Zhang Nianhui *.Sensitivity of prestaining RNA with ethidium bromide before electrophoresis and performance of subsequent Northern blots using heterologous DNA probes. Mol Biotechnol, 2013, 54: 204-210, (SCI, IF=1. 60)
21. Wang Jing-Zhang, Xi Lei, Zhu Guo-Fei, Han Yong-Guang, Luo Yue, Wang Mei,Du Lin-Fang*. The acidic pH-induced structural changes in Pin1 as revealed by spectral methodologies. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2012, 98: 199-206. (SCI, IF=1. 60)
22. Feng, Cao Hou-Li,Du*. Apoptosis induced by genipin in human leukemia K562 cells: involvement of c-Jun N-terminal kinase in G2/M arrest.. 2011,. (SCI, IF=1. 60)
23. Wang Jing-Zhang, Lin Tao, Teng Teng, Xie Si-Si, Zhu Guo-Fei,Du Lin-Fang *. Spectroscopic studies on the irreversible heat-induced structural transition of Pin1. Spectrochimica Acta Part A, 2011, 78: 142-147. (SCI, IF=1. 60)
24.; Wang Jing-Zhang;; Huang Xin-He;;*.Heat treatment-induced functional and structural aspects of Mus musculus TAp63γ. Journal of Molecular Structure, 2011, 996:42-47. (SCI, IF=1. 60)
25. Lin Tao, Wang Jingzhang, Li Lingjin,Du Linfang *. Investigation of the secondary structure and interaction of hPin1 with cobalt ion by Fourier transform infrared spectroscopy. Asian Journal of Chemistry, 2011, 23: 1041-1043. (SCI, IF=0. 60)
26. Cao Houli,# Feng Qian,# Xu Wei, Li Xiaorong, Kang Zhuang, Ren Yanqin,Du Linfang*. Genipin induced apoptosis associated with activation of the c-jun nh2-terminal kinase and p53 protein in hela cells. Biol. Pharm. Bull. 2010, 33:1343-1348. (SCI, IF=1. 81)
27.Wang Jing-Zhang, Lin Tao, Zhu Guo-Fei,Du Lin-Fang*.Stability of Pin1 as revealed by thermal and spectroscopic studies. Journal of Molecular Structure, 2010, 975: 310-316. (SCI, IF=1. 60)
28. Effects of inorganic mercury on the conformation and activity of glutathione s-transferase from Schistosoma Japonicum. Asian Journal of Chemistry, 2010, 22:3458-3468. (SCI, IF=0. 25)
29. Stability study on lectin from Dolichos Purpureus seeds by means of fluorescence and circular dichroism.Asian Journal of Chemistry, 2010, 22:3469-3478. (SCI, IF=0. 25)
30. Xu Tao, Gu JianLei, Zhou Yan*,Du LinFang*. Improving detection of differentially expressed gene sets by applying cluster enrichment analysis to Gene Ontology. BMC Bioinformatics, 2009, 10:240 (SCI, IF=3. 43)
31.蒋佳宏,王东,胡源,高秀,杜林方*.光照与温度对拟南芥tak基因表达与LHC磷酸化的影响. Prog Biochem Biophys, 2009, 36: 1202-1207. (SCI, IF=0. 19)
32. Li Jia Wang Jing Zhang. Expression and purification of tobacco PR-1a protein for function analysis. Asian Journal of Chemistry , 2009, 21: 3697-3707. (SCI, IF=0. 21)
33. Kang Zhuang, Jiang Jiahong, Wang Dong, Liu Ke,Du Linfang*. Kunitz-type trypsin inhibitor with high stability from spinacia oleracea L. seeds. Biochemistry (Moscow), 2009, 74: 102-109. (SCI, IF=1. 32)
34. Liu Xin,Du Lin Fang*, Huang Xin He, Zhu De Yan, Li Jia And Wang Jing Zhang. Purification of recombinent p19ARF: Protein interaction and stability analysis. Asian Journal of Chemistry. 2009, 21:279-286. (SCI, IF=0. 21)
35. Hu Yuan, Gao Xiu, Jiang Jiahong, Ran Yan,Du Linfang*. Effects of temperature on the expression of STN7 and phosphorylation of LHCII proteins in Arabidopsis thaliana. Chinese Science Bulletin 2008, 53:(17)2646-2651. (SCI, IF=0. 68)
36. Yuan Han, Han Lu, Du Li-Bo, Tian Qiu, Liu Ke,Du Lin-Fang*，Liu Yang*. Spin trapping-ESR studies on the superoxide anion radical generated in photosystem II of unicellular green alga chlamydomonas reinhardtii. Progress in Biochemistry and Biophysics. 2008, 35(9): 1065~1069.(SCI, IF=0. 21)
37. Xu Tao,Du LinFang*, Zhou Yan*. Evaluation of GO-based functional similarity measures using S. cerevisiae protein interaction and expression profile data. BMC Bioinformatics 2008, 9:472. (SCI, IF=3. 78)
38. Wang Jing, Tang Fushan, Li Feng, Lin Juan, Zhang Yinghui, Du Linfang, Zhao Xiaojun, The Amphiphilic Self-Assembling Peptide EAK16-I as a Potential Hydrophobic Drug Carrier. Journal of Nanomaterials. Volume 2008 (2008).
39. Guo Junwei, Guo Jinkui，Zhao yun，Du Linfang*. Changes of photosystem II electron transport in the chlorophyll-deficient oilseed rape mutant studied by chlorophyll fluorescence and thermoluminescence. Journal of Integrative Plant Biology 2007, 49 (5): 698−705 (SCI, IF=0. 67)
40. Liao Hai, Ren Wei, Zhuang Kang, Jiang Jia-Hong, Zhao Xiao-Jun,Du Lin-Fang*. A trypsin inhibitor from Cassia obtusifolia seds: isolation, characterization and activity againstPieris rapae.Biotechnology Letters, 2007, 29: 653–658 (SCI, IF=1. 21)
41. Qin Tingting, Fu Jingfeng, Zhang Nianhui,Du Linfang*. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b-deficient mutant and its wild type oilseed rape during senescence. Plant Science, 2006, 293-299 (SCI, IF=1. 63)
42.Wei Huimin, Guo Junwei, Zhang Shuang, Huang Bo,Du Linfang*, The presence of phosphorylation form of D1 protein in its cross-linked aggregates in high light treated spinach leaves in vivo. Chinese Science Bulletin, 2006, 51:69-74. (SCI, IF=0. 72)
43. Wang Tao, Zhang Nianhui,Du Lingfang*. Isolation of RNA of high quality and yield from Ginkgo biloba leaves. Biotechnology Letters, 2005, 27: 629–633. (SCI, IF=1. 11)
44. Zhang NianHui, Wei Zhengquan, He JunXian, Liang Houguo,Du Linfang*. An efficient and economic method for preparation of high quality plant RNA. Prog. Biochem. Biophys. 2004，31:947- 950. (SCI, IF=0. 21)
45. Li Chi-Jun, Liang Hou-guo, Du Lin-fang, Wang Rui. Studies on the relationship between cyanide-resistant respiration and expression of alternative oxidase in mung bean using antibodies prepared by synthetic polypeptide. Science inChina(series C), 2001, 44: 66-72. (SCI)
46.李炯，杜林方*。一种快速检测植物类囊体膜蛋白体内磷酸化的方法。生物化学与生物物理进展, 2001, 29:740-743. (SCI)
47.周昊,杜林方*,朱晓峰.用于植物光系统II蛋白酶检测的活性染色方法。生物化学与生物物理进展, 2002, 30:651-654. (SCI)
48.赵云，杜林方*，杨胜洪，李世崇，张义正. Chloroplast composition and structural differences in a chlorophyll-reduced mutant of oilseed rape seedlings. Acta Botanica Sinica, 2001, 42: 877-880. (SCI)
获奖:
“植物光系统II的结构、功能与活性调节”，2000年教育部中国高校自然科学一等奖
“植物主要膜系统电子传递的生理功能与活性调节”，2003年度教育部自然科学二等奖
2004年被国务院授予国家政府特殊津贴
被选为国家教委“跨世纪优秀人才计划”
入选教育部首批“高等学校骨干教师计划”
宝钢优秀教师奖
